红肉小果型番木瓜品种‘美中红’体胚的诱导

Induction of Somatic Embryos from Chinese Cultivar 'Meizhonghong' of Papaya(Carica papaya L.) with Red Flesh

用红肉小果型番木瓜品种‘美中红’为外植体，探讨不同成熟度的幼胚、不同浓度2,4-D和培养条件对其体胚的诱导以及体胚形成过程的结果表明：以子叶和内外种皮都为白色且个体较大的番木瓜幼胚（90～120d）在含10mg·L^-1 2,4-D的培养基中和黑暗条件下诱导愈伤组织的效果最佳，愈伤组织的诱导率随着2冉D浓度的增加而增加．番木瓜愈伤组织最先发生于形态学上的胚根下端，体胚多发生于形态学上的胚芽上端。

The effects of different maturities of papaya （Carica papaya） embryos, different concentrations of 2,4-D and culture conditions on embryogenesis of Chinese cultivar ＇Meizhonghong＇ and the progress of somatic embryogenesis were studied. The results showed that the bigger embryos （90-120 d） with white cotyledon and episperm cultured on MS induction medium supplemented with 10 mg·L^-1 2,4-D were best for induction of calli. The callus induction rate increased with the increase of concentration of 2,4-D from 0-10 mg·L^-1. Calli formation occurred first from epidemic cells of radicle and somatic embryo mostly occurred from the calli of plumula.