粒-巨噬细胞集落刺激因子对大鼠降钙素水平的影响

The effect of granulocyte-macrophage colony-stimulating factor on rat calcitonin

目的探讨粒-巨噬细胞集落刺激因子(GM-CSF)对大鼠降钙素水平的影响。方法给动物腹腔注射不同剂量的GM-CSF,然后用RIA试剂盒检测血中降钙素水平,同时测血中钙、磷及碱性磷酸酶。结果血清钙水平各实验组均比对照组降低,但仅大剂量组与对照组间差异有统计学意义(P<0.05);雌性大鼠血清降钙素水平各实验组均比对照组明显降低(P<0.05);实验组的血清磷均比对照组升高,但无统计学意义(P>0.05);血清碱性磷酸酶未见有显著的组间差异。结论50μg/kg的GM-CSF可以引起SD大鼠血清钙下降;10μg/kg和50μg/kg的GM-CSF均可以引起雌性大鼠的血清降钙素降低,但对血磷、血清碱性磷酸酶无显著影响。

Objective To investigate the effect of granulocyte-macrophage colony-stimulating factor （GM- CSF） on rat calcitonin （CT）. Methods A total of 48 SD healthy rats were randomly divided into control group, low GM-CSF （10 μg/kg · d） and high GM-CSF dosage （50μg/kg · d） groups. The rats in experimental groups were continuously treated by an intraperitoneal application of GM-CSF for over 7 days. The level of calcitonin was measured by radioimmunoassay（RIA）, and the serum calcium （Ca）, ALP and phosphorus（P） were examined by biochemical method. Results The level of the serum calcium in high-dosage GM-CSF group was lower than that in the control group （P〈0. 05）; the level of the serum calcitonin in the low- and high-dosage GM-CSF treated female rats was lower than that in the control group （P〈0.05）; the level of the serum phosphorus（ P） in the experimental group was higher than that in the control group（P〉0.05）. Conclusion The high dosage GM-CSF can decrease the serum calcium; the low- and high-dosage GM-CSF can decrease the serum calcitonin, but has no effect on the level of serum phosphorus and ALP.