一氟磷酸谷酰胺及αD3对去势大鼠下颌骨骨密度的影响

Effects of Monofluorphosphate and αD3 on the Bone Mineral Density of Mandibular of Ovariectomy Rats

目的探讨一氟磷酸谷酰胺及αD3对去势大鼠下颌骨骨密度的影响。方法将3月龄SD雌性大鼠39只随机分成4组:假手术组(SHAM组),去卵巢不处理组(OVX组),去卵巢单用一氟磷酸谷酰胺组(MFP组),去卵巢一氟磷酸谷酰胺与αD3联合用药组(MFP+αD3组)。在大鼠去势后6周,MFP组将一氟磷酸谷酰胺溶于蒸馏水后灌胃,MFP+αD3组将一氟磷酸谷酰胺和αD3溶于蒸馏水后灌胃。每天1次,每周6次,连续12周。采用HPIAS10000型图像分析系统进行下颌骨组织形态测量,采用双能X线测量股骨和下颌骨的骨密度。结果OVX组与SHAM组、MFP组、MFP+αD3组相比,下颌骨骨小梁面积、骨小梁宽度和松质骨面积明显减少,骨小梁间隔增大,股骨骨密度减小,而SHAM组、MFP组、MFP+αD3组三者之间无明显差异;OVX组、MFP+αD3组的下颌骨骨密度较SHAM组升高,差异有显著性,而MFP组与SHAM组间无显著性差异。结论一氟磷酸谷酰胺及αD3能促进去势大鼠下颌骨骨量的增加,下颌骨骨密度的改变与四肢骨不一致。

Objective To investigate the effects of monofluorphosphate and αD3 on the bone mineral density of mandibular of ovariectomy rats. Methods 39 female Sprague-Dawley rats about 90 days of age were randomly divided into the following treatment groups and treated for 12 weeks： SHAM group, OVX group, MFP group, MFP＋αD3 group. Rats of MFP group received monofluorphosphate by gastric feeding. Rats of MFP＋αD3 group received monofluorphosphate and αD3 by gastric feeding. The femur and the mandibles were collected for histomorpbometry and bone mineral density measurement. Results The bone area, bone thick, and bone mineral density of femur in the OVX group were significantly lower than those of other groups, while the trabecular separation of the OVX group was significantly higher than that of other groups. The bone mineral density of mandible was increased in OVX group and MFP＋αD3 group compared with SHAM group. Conclnsion Monofluorphosphate and αD3 can obviously increase the mass of mandible. The bone mineral density of femur decreased after ovariectomy while it increased in mandibular.