摘要
用杨盘二孢菌的激发子粗提液,与高度抗黑斑病的I-72杨和高度感染黑斑病的加杨悬浮细胞互作,测定杨树悬浮细胞的生物化学变化。结果表明:I-72杨在活性氧的释放和ΔpH的变化两方面均明显高出加杨;膜脂过氧化过程,从量的方面看,二者几乎没有差异,只是I-72杨的膜脂过氧化高峰期要早于加杨1h。这些研究可以为杨树的抗病机理和杨树抗病基因的研究提供基础。
The biochemical changes of suspension cell of the susceptible poplar and the resistant one through the interaction between suspension cell of poplar and the crude elicitor of Marssonina brunnea were determined. The results are as below: the production of active oxygen and the △pH of suspension cell of Ⅰ - 72 poplar were higher than Populus canadensis in whole process. There was no distinct difference on the quantity in the membrane lipid peroxidation (MDA) of suspension cell of P. canadensis and Ⅰ - 72, but the MDA rush hour in Ⅰ - 72 appeared one hour earlier than in the P. canadensis poplar. These could provide a foundation to understanding the resistant mechanism and the resistant gene of poplar.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2007年第1期68-71,共4页
Scientia Silvae Sinicae
基金
国家自然科学基金项目"杨树和杨盘二孢菌互作系统中激发子及其功能研究"(30271081)