摘要
目的研究转化生长因子-β1(TGF-β1)所诱导的上皮向间叶转化对胰腺癌侵袭的意义。方法采用免疫组织化学的方法检测29例胰腺癌组织中TGF-β1的表达,并与临床病理资料作对照分析,同时体外通过TGF-β1诱导胰腺癌细胞株Panc-1,观察上皮向间叶转化(EMT)的发生情况以及对细胞侵袭能力的影响。结果在29例胰腺癌组织中有12例TGF-β1呈阳性表达(41.4%),TGF-β1的阳性表达和肿瘤的侵袭范围(P=0.035)以及淋巴结转移(P=0.047)都存在着相关性。TGF-β1在体外可以诱导Panc-1发生明显的EMT以及侵袭能力的增加。结论TGF-β1可以通过诱导胰腺癌细胞发生上皮向间叶转化和肿瘤细胞侵袭能力的增加,进而导致了胰腺癌高度侵袭性的生物学行为。
Objective To study the significance of EMT induced by TGF-β1 on pancreatic cancer （PC） invasion and metastasis. Methods TGF-β1 expression was detected in 29 cases of human PC by using immunohistochemistry and the results were compared with clinicopathological data. The effects of TGF-β1 on the phenotype and invasion of PC cell line Pane-1 were also investigated. Results TGF-β1 was detected in 12 cases （41.4%） of PC. There was a significant correlation between the expression of TGF-β1 and lymph node involvement （P=0. 047）, the depth of invasion （P=0. 035）. TGF-β1 promoted obvious EMT of Pane-1 cell lines and greatly enhanced the invasion ability. Conclusion TGF-β1 may promote the malignancy of PC by triggering EMT.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期67-70,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目(No.30571817)
关键词
胰腺癌
转化生长因子-Β1
上皮向间叶转化
pancreatic cancer
transforming growth factor β1
epithelial to mesenchymal transition
