子宫颈病变组织中血小板反应素-1的表达及其与血管生成的关系

Expression of TSP-1 in Cervical Lesions and Its Relationship with Angiogenesis

目的探讨各级子宫颈上皮内瘤样病变（CIN）及早期子宫颈鳞状细胞癌组织中血小板反应素-1（TSP-1）的表达及与微血管密度（MVD）的关系。方法采用免疫组织化学SP法检测在10例正常子宫颈组织、13例CINⅠ、22例CINⅡ～Ⅲ及30例子宫颈癌组织中TSP-1的表达情况及MVD。结果正常子宫颈、CINⅠ、CINⅡ～Ⅲ及子宫颈癌组织中TSP-1表达强度及MVD计数分别为（3．7±1．5）、（3．0±1．4）、（1．9±1．2）、（1．7±1．0）及（6．8±1．9）、（13．0±3．6）、（22．7±4．4）、（31．0±5．3）。TSP-1主要表达在正常子宫颈组织基底层。子宫颈癌和CINⅡ～Ⅲ组织中TSP-1的表达明显低于正常子宫颈和CINⅠ组织（P〈0．01）。正常子宫颈与CINⅠ，CINⅡ～Ⅲ与子宫颈癌组之间TSP-1表达差异无显著性意义（均P〉0．05）。MVD随着子宫颈病变级别升高而增加。子宫颈病变中TSP-1的表达与MVD呈显著负相关（rs=-0．603，P〈0．05）。结论TSP-1低表达可能参与了子宫颈癌血管生成过程，联合检测TSP-1和MVD可能在子宫颈癌的早期筛查、诊断、治疗中具有一定意义。

Objective To study the expression of thrombospondin-1 （TSP-1） and microvessel density （MVD） in cervical intraepithelial neoplasia （CIN） and cervical cancer and to analyze their correlations. Methods Immunohistochemical SP method was employed to detect TSP-1 and MVD in 10 cases of normal cervix, 13 cases of CINⅠ , 22 cases of CIN Ⅱ-CIN Ⅲ and 30 cases of cervical cancer. Results The mean TSP-1 scores and MVD counts for normal cervices, CINⅠ , CIN Ⅱ -Ⅲ and cervical cancer were （3.7±1.5）, （3.0±1.4）, （1.9±1.2）, （1.7±1.0）and （6.8±1.9）, （13.0±3.6）, （22.7±4.4）, （31.0±5.3） respectively. TSP-1 was mainly expressed at the normal cervical basal epithelial cell layer. The expression of TSP-1 in cervical cancer and CIN Ⅱ -Ⅲ group was significantly lower than in normal cervix and CINⅠ group （P〈0. 01）. But there was no significant difference in the expression of TSP-1 between normal cervix and CINⅠ , CIN Ⅱ -Ⅲ and cervical cancer （P〉0.05）. The MVD counts were gradually increased with the progressive cervical lesions. TSP-1 expression was negatively correlated to the MVD count （rs = -0. 603, P〈0.05）. Conclusion The decreased expression of TSP-1 in cervical lesions may be involved in cervical cancer angiogenesis. TSP-1 and MVD may be used as biomarkers in the screening, diagnosis and treatment of cervical cancer.