摘要
目的比较清开灵不同制剂的高效液相色谱指纹图谱。方法选择NUDEOSIL-C18色谱柱(4.6 mm×250 mm,5μm),流动相(A)甲醇-(B)0.5%醋酸梯度洗脱,运行时间65 min,检测波长255 nm,测定清开灵注射液的指纹图谱。结果以黄芩苷作为参照峰,得出各色谱峰的相对保留时间和相对峰面积。结论清开灵不同制剂的指纹图谱相差较大,提示不同的生产工艺可能对其成分及含量有较大的影响。
Objective To compare the fingerprint chromatogram of different preparations of Qingkailing Compound. Methods HPLC with NUDEOSIL - C18 column was used, the mobile phase was (a) methanol, (b)0.5% acetate buffer and the detection wavelength was at 255nm. Results Peaks were indicated on the HPLC-fingerprint of different preparations of Qingkailing Compound. The relative retention time and relative peak area were obtained with itself peak. Conclusion The fingerprint chromatogram of different preparations of Qingkailing Compound are noticeably different, which indicates different manufactnre technology affects ingredients and contents of Qingkailing preparations.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第2期442-444,共3页
Lishizhen Medicine and Materia Medica Research
关键词
清开灵
指纹图谱
高效液相色谱
Qingkailing Compound
Fingerprint chromatogram
HPLC