清开灵干预新生大鼠缺氧缺血脑皮质白介素-6及Fas-L蛋白的实验研究

Effects of QingKaiLing on the Radioimmunologic Concentrations of IL-6,the Expression of Fas-L Protein in the Cortex in Neonatal Rats after Hypoxic-ischemic Brain Damage

目的探讨缺氧缺血脑损伤对新生大鼠HIBD脑皮质IL-6放射免疫浓度,Fas-L蛋白表达的影响,及清开灵对新生大鼠HIBD的干预治疗作用以及有关分子机制。方法建立HIBD动物模型后分为放射免疫法测定组和免疫组化测定组,每组又分为清开灵干预组、生理盐水对照组、假手术组,根据检测需要,在缺氧缺血后24 h,3 d,7 d作IL-6脑皮质匀浆放射免疫浓度测定、Fas-L蛋白免疫组化SP法测定。结果①脑皮质组织匀浆IL-6放射免疫浓度(pg/ml)假手术组、生理盐水组、清开灵干预组:24 h依次为50.1±6.2,56.3±7.1,51.3±6.8;3 d依次为50.6±6.4,250.3±11.2,78.1±8.3;7 d依次为51.4±6.6,345.7±12.3,81.2±9.2,生理盐水组与另两组分别比较,24 hP均>0.05,无明显变化;3,7d生理盐水组增加明显?P<0.05)清开灵干预组降低明显?P<0.05)。②③④左脑皮质Fas-L蛋白表达阳性率假手术组、生理盐水组、清开灵干预组:24 h依次为8.04±3.50,120.20±6.10,21.10±5.10;3 d依次为8.20±3.10,87.6±5.90,13.40±4.20;7 d依次为8.01±2.80,8.32±2.90,8.26±2.83;生理盐水组与另两组分别比较,7 dP均>0.05,无明显变化;24 h,3 d增多明显(P<0.05),清开灵干预组降低明显(P<0.05)。结论①新生大鼠HIBD后24 h,3 d,7 d脑皮质IL-6含量逐渐增高。②清开灵能降低新生大鼠HIBD脑皮质IL-6的产生量。③新生大鼠HIBD后,结扎侧脑皮质fas-L蛋白表达增高。④清开灵能抑制新生大鼠HIBD结扎侧脑皮质fas-L蛋白的高表达。

Objective To explore the immunologic changes of interleukin -6 （IL- 6） , the expression of fas- L protein of cortex in newborn rats after HIBD and the effects of QingKaiLing on them. Methods To divide animals for experiment into groups： IL - 6 test group： Animals were divided into sham group, NS group and QingKailing group, IL- 6 radioimmunologic concentrations of cortex were measured at 24 h, 3d and 7d after hypoxia ischemia by radioimmunoassay. Immunohistochemistry. group： Animals were divided into sham group, NS group and QingKaiLing group. The changes of expression of fas - L protein were detected at 24h, 3d and 7d after hypoxia ischemia by immunohistochemistry. Results Comparing with the sham group, the concentration of IL -6 and the expression of fas -L protein （at 24h, 3d and 7d） increased obviously in the ligated side of the brain cortex in NS group （P 〈0.05 ） . Comparing with NS group, they were on the contrary in QingKaiLing group（P 〈 0.05）. Conclusion The concentration of IL-6, the expression of fas -L protein become higher rapidly after HIBD. QingKaiLing can inhibit the production of IL -6 and the expression of fas - L protein.