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CO_2对卵巢癌细胞株SKOV3抗凋亡因子XIAP和PED/PEA-15表达的影响 被引量：3

Effects of carbon dioxide on the expressions of XIAP and PED/PEA-15 in human ovarian cancer cell line SKOV3
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摘要 目的:探讨CO_2气腹对卵巢癌细胞株SKOV3抗凋亡因子XIAP和PED/ PEA-15表达的影响。方法:建立体外模拟CO_2气腹环境,将人卵巢癌细胞株SKOV3分别暴露于不同压力(8～12mmHg)的CO_2环境中,持续1、2、3h后,脱离CO_2环境,放入常规CO^2培养箱中培养,分别于培养24、48、72h后用实时荧光定量PCR法及免疫细胞化学法检测各组SKOV3细胞XIAP、PED/PEA-15 mRNA和蛋白的表达。结果:SKOV3细胞置于不同的CO_2气腹环境后,与对照组比较,SKOV3细胞XIAP、PED/PEA-15的表达明显增加(P＜0．05),并且与CO_2压力和作用时间有关。结论:CO_2气腹环境可促进SKOV3细胞中抗凋亡因子XIAP、PED/PEA-15表达增加,从而抑制细胞凋亡。 Objective： To explore the effects of carbon dioxide on the expressions of antiapoptosis factors XIAP and PED/PEA-15 in human ovarian cancer cell line SKOV3. Methods：Human ovarian cancer cell line SKOV3 was put in analogous CO2 pneumoperitoneum environment. Realtime FQ-PCR and immunocytochemical staining were used to examine the expressions of XIAP and PED/PEA-15 of these cells after exposure to different pressures and persistence of CO2. Results： CO2 could significantly increase the expressions of XIAP and PED/PEA- 15 （P 〈0.05）. The expressions of XIAP and PED PEA-15 were related with the pressure and persistence of CO2. Conclusion： CO2 can suppress apoptosis capacity of SKOV3 cell by upregulating the expressions of antiapoptosis factors XIAP and PED/PEA-15.
作者 张晓慧 张爱荣 张萍 王亚平 许馥郁
机构地区 山东大学第二医院妇产科 山东大学第二医院病理科
出处 《现代妇产科进展》 CSCD 北大核心 2007年第1期25-28,I0003,共5页 Progress in Obstetrics and Gynecology
关键词 卵巢肿瘤 二氧化碳 细胞凋亡 XIAP PED/PEA-15 Ovarian neoplasms Carbon dioxide Apoptosis
分类号 R737.31 [医药卫生—肿瘤]
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参考文献9

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二级参考文献11

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现代妇产科进展
2007年 第1期

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