摘要
目的:研究雷洛昔芬和依普黄酮对切除成熟卵巢大鼠骨干生物力学性能的影响,探讨此两种药物治疗绝经后骨质疏松症的疗效。方法:选用6月龄未交配健康雌性Sprague-Dawley大鼠50只,随机分为5组,每组10只:(1)假手术组(sham);(2)骨质疏松组(OVX);(3)OVX加戊酸雌二醇组(Valerate Estriol,E_2;0．8mg·kg^(-1)·d^(-1));(4)OVX加雷洛昔芬组(Raloxifene,RLX;5mg·kg^(-1)·d^(-1));(5)OVX加依普黄酮组(Ipriflavone,IPR; 100mg·kg^(-1)·d^(-1));于去势手术3周后开始按分组设计喂药(灌胃),治疗3个月后处死。取大鼠右股骨及第三腰椎测定骨密度;然后对右股骨中段进行三点弯曲试验,测定多个股骨干生物力学指标。结果:(1)骨密度:OVX组椎骨及股骨干骺端密度明显下降,使用E_2和RLX能够显著提升骨密度;但股骨干密度各组间差异无统计学意义;(2)骨干生物力学性能:三点弯曲实验结果显示,OVX组股骨干生物力学指标较sham组明显下降,用RLX和IPR治疗与E_2一样可阻止这种变化(P＜0．05)。结论:雷洛昔芬和依普黄酮能阻止卵巢切除所致的成熟雌性大鼠股骨干(皮质骨)生物力学性能受损。
Objective- To evaluate the effect of treatment with raloxifene and ipriflavone on diaphyseal biomechanical quality in mature ovariectomized （OVX） rats. Methods： Sixmonth-old Sprague-Dawley（SD） rats were divided randomly into five groups （n = 10）. Sham- operated and control OVX groups received vehicle, whereas other groups received Valerate Estriol （ E2 0.8mg· kg^-12 d^-1 ）, Raloxifene （ RLX 5mg · kg^-1· d^-1 ） or Ipriflavone （ IPR ; 100mg · kg^-1 ·d^-1 ） for 3 months, respectively. Then the animals were anesthetized and an autopsy was performed. BMD of the right femur and the lumber vertebrae L3 of rats were measured with a DEXA. Femoral diaphyseal biomechanical properties were evaluated by three-point bending test. Results：Treatment with raloxifene and E2 significantly prevented ovariectomy-induced BMD loss in femoral epiphysis and lumber vertebra L3. BMD of femoral diaphysis was not different between groups, but bone biomechanical quality of femoral diaphysis of OVX rats decreased significantly ,treatment with raloxifenen, ipriflavone and E2 significantly prevented ovariectomy-induced loss of bone biomechanical quality of femoral diaphysis to a similar extent. Conclusion： Ralox-ifene and ipriflavone can prevent ovariectomy-induced deterioration of biomechanics properties of femoral diaphysis in mature ovariectomized （OVX） rats.
出处
《现代妇产科进展》
CSCD
北大核心
2007年第1期46-49,共4页
Progress in Obstetrics and Gynecology
