革兰阴性杆菌高产AmpC酶的常规检测方法

Study on routine detection of high yield AmpC-blactamase of gram-negative bacillus

目的探讨革兰阴性杆菌高产AmpC酶的常规检测方法。方法用头孢西丁三相试验(甲法)、双纸片协同法(乙法)和酶提取物三维试验法(丙法)检测临床分离的155株革兰阴性杆菌高产AmpC酶产生的情况。结果在155株革兰阴性杆菌中,甲法检测出高产AmpC酶65株,检出率为41.9%,乙法检测出57株,检出率为36.8%,丙法检测出55株,检出率为35.5%。甲法与丙法符合率为93.5%,乙法与丙法符合率为98.7%。乙法当纸片间的距离从30mm缩小到20mm时,高产AmpC酶的检出率从29.7%提高到36.8%。结论乙法不需用特殊仪器,操作简便,结果可靠,可作为临床实验室常规检测AmpC酶的方法。

Objective To study the routine detection of high yield AmpCblactamase of gram-negative bacillus. Methods Cefoxitin three-dimensional test, double-disc synergy test and enzyme extraction three-dimensional test were performed to detect high yield AmpC- blactamase of gram-negative bacillus. Results In 155 strains of gram-negative bacillus, there were 65 strains with high yield AmpC-blactamase by Cefoxitin three-dimensional test （41.9%）, 57 strains by double-disc synergy test （36.8%） and 55 strains by enzyme extraction three-dimensional （35.5%） respectively. The result of double-disc synergy test was 98.7% similar to that of enzyme extraction three-dimensional. In double-disc synergy test with decreasing the size between the discs from 30mm to 20mm, the detecting rate of high yield AmpC-blactamase was improved from 29.7% to 36.8%. Conclusions Double-disc synergy test is simple, correct, demanding no special equipments and fit for clinical routine detection of AmpC-blactamase.