转Bt－基因抗虫棉棉子安全性评价──鲤鱼慢性毒性试验

An Assessment on the Safety of Cottonseed Meal from Bt-trans- genic Cotton Plant on the Chronic Toxicity Test with Carp

为评价转Ｂｔ－基因抗虫棉脱酚后棉仁粉用作鱼饲料的有效性和安全性，本文进行了１２用的鲤鱼慢性毒性试验。对鲤鱼的增重率、特定生长率、饵料系数、成活率等营养学指标的观察以及对鲤鱼的血像、生化、遗传、病理组织等毒理学指标的分析结果表明，就上述所观察的指标，含抗虫棉棉仁粉和含泗棉３号棉棉仁粉之间均无显著性差异。证明转Ｂｔ－基因抗虫棉棉仁粉经脱棉酚处理后，对水生动物无毒性作用，与常规棉棉仁粉一样可用作水生动物饲料的蛋白质资源。

To evaluate the efficiency and safty of cottonseed (gossypol<0.05%)contain- ing Bt-protein from Bt-transgenic cotton plant resistant to insect,as feed for fish,a 12- week chronic toxicity test was conducted with Cypinus carpio using diets containing 30%(by weight) processed cottonseed meal from the insect resistant and normal cotton plant. No deleterious effects on carp growth and development were observed by investigating be- havior,weight gain rate,feed efficency and survive rate of fish,No significant differences(P<0.05)in the blood indexes such as WBC、Hb,GPT,BUN and the rate of micronucle- us of red corpusles were found.NO pathological changes were found in organs such as liv- er,kidey,intestines,heart ect.of carp in both test groups and control groups byhistopathology examination,Also,no differences(P>0.05)were observed in the organrelative weight between test groups and the control groups. These results demonstrate thesafety of the cottonseed from Bt-transgenic cotton plant to water animals when used asfishery feed resources.