维生素E对老化肝脏枯否细胞能量代谢改变的防治研究

EFFECT OF AGING ON KUPFFER CELL’S ENERGY METABOLISM AND THE PROTECTIVE FFECT OF VITAMIN E

测定了不同月龄组（６、１２、１８和２４）大鼠离体肝脏枯否细胞（ＫＣ）葡萄糖利用能力和线粒体的能量代谢状态，以及维生素Ｅ（ＶＥ）对这种状态的影响。结果表明，１８月龄组和２４月龄组肝脏ＫＣ葡萄糖利用能力和线粒体能量代谢明显低于６月龄组，前者分别为６月龄组的８０．７％和６４．９％，后者分别为８０．５％和６７．９％。１８月龄和２４月龄ＶＥ治疗组葡萄糖利用能力和线粒体能量代谢状态均有明显改善，并以１８月龄组最明显，两者分别为正常对照的９４．７％和９７．３％。结果提示，老化对ＫＣ葡萄糖利用能力和线粒体能量代谢具有明显的负影响，ＶＥ对这种影响具有较好的预防作用。

sing Rhodamine 123 fluorescence density and rate of glucose utilizationas parameters,we measured the mitochondrial energy metabolism status andthe glucose utilization capacity of rat liver Kupffer cells(KC)of variousages(6mo,12mo,18mo and 24mo)and the effect of vitamine E(VE)pre-treatment on them.The results indicated that KC energy metabolism of 18mo and 24 mo groups were significantly decreased compared with that of6mo group.Such decrease could be related by VE pretreatment(500mg·kg￣(-1)·Wk￣(-1)×13wks),especially that of 18 mo group. on KC energy metabolism which canbe prevented in some degree by VE pretreatment.