71例急性早幼粒细胞白血病的MICM分型研究

Study on MICM classification of acute promyelocytic leukemia in 71 cases

本文对７１例形态学（Ｍｏｒｐｈｏｌｏｇｙ）诊断为急性早幼粒细胞白血病（ＡＰＬ）的初发患者骨髓标本同时进行了免疫学（Ｉｍｍｕｎｏｌｏｇｙ）、细胞遗传学（Ｃｙｔｏｇｅｎｅｔｉｃｓ）和分子生物学（Ｍｏｌｅｃｕｌａｒｂｉｏｌｏｇｙ）（简称ＭＩＣＭ）的分型研究。典型的ＡＰＬ免疫学粒细胞抗体中ＣＤ３３、ＣＤ１３有很高的表达；染色体核型为ｔ（１５；１７）（ｑ２２；ｑ１１～２１）；ＲＴ／ＰＣＲ检测可见ＰＭＬ／ＲＡＲα融合基因。这种ＡＰＬ占本组病例的９５．８％。另二例有其他的染色体易位和基因的异常。提示ＡＰＬ是不均一性疾病。

Bone marrow samples from 71 cases of acute promyelocytic leukemia (APL) were studied for their morphology, immunology, cytogenetics and molecular biology(MICM). The result showed that there were CD33, CD13 high expression, chromosomal translocation t(15;17) (q22; q11-21) and PML/RARa fusion gene in 69 of the 71 cases. The two remaining cases were found atypical chromosomal translocation and gene heterogeneity. These studies suggested that APL is not a homogenous disease.