毛细管气相色谱法测定抗鼻咽癌口服液中斑蝥素的含量

Quantification of cantharidin in an oral herbal liquid against nasopharyngeal carcinoma by capillary gas chromatography

目的：建立了抗鼻咽癌口服液中斑蝥素的含量测定方法。方法：选用DB-5弹性石英毛细管柱（30m×0．25mm，0．25um）；检测器为FID检测器；柱温为150℃，保持25min；气化室温度为250℃；检测器温度为320℃；载气为氦气；柱头压力为140kPa；分流比为30：1。结果：斑蝥素在0．02～0．24ug范围内呈良好的线性关系，r=0．9998（n=6）；样品的平均加样回收率为97．24％．RSD=1．35％（n=6）。结论：本法简便、快速．可用于抗鼻咽癌口服液的质量控制。

Objective：To establish a capillary gas chromatography（GC）method for quantification of cantharidin in an oral herbal liquid against nasopharyngeal carcinoma （kangbiyanai oral liquid）. Methods：The GC assay was performed with a DB-5 capillary column（30 m × 0.25 mm,0.25um） with a FID detector. The temperature of column temperature was at 150℃, the gasifying chamber at 250℃ and the FID detector at 320℃. Helium was used as the carrier gas. The pressure at the column head was 140 kPa. Results： The linearity of cantharidin concentration curve was within 0.02 -0.24 ug（ r = 0.999 8 ）. The mean recovery of cantharidin was 97.24% （ RSD = 1.35% , n = 6）. Conclusion： A rapid and reliable GC method is attainable in the quality control of kangbiyanai oral liquid.