氦氖激光穴位针刺对脾脏作用的电镜观察

Effect of HE-NE Laser Acupuncture on the Spleen in Rats

本研究应用小功率激光穴位针刺，脾笔毛动脉及血窦扩张，内皮间隙加宽，红髓渗入大量红、白细胞，此表示脾血流加速，改善其血液及微循环。T淋巴细胞胀大，核糖体及线粒体加多，B淋巴细胞分化的前及成熟型浆细胞数目加多，内质网密集，池扩张，合成免疫球蛋白机能增强。即针刺促增脾的细胞免疫及体液免疫机能。巨噬细胞含大量吞噬体、空泡及多泡体。交错突细胞活性增强，表示针刺引起脾抗原提呈细胞摄入大量抗原物质，输入T淋巴细胞，促其活化及增殖。本文还讨论针刺与目前发展的神经、内分泌和免疫系统网络的相互调控作用，说明激光穴位针刺所引起脾的免疫机能提高，可能与此网络调节有关。

The splenic sinuses, lymphocytes and antigen presenting cells in the spleen of rat stimulated by He-Ne laser acupuncture were observed by using TEM to investigate the ultrastructural changes of them.The width of endothelial interstice was increased, hence a lot of blood cells oozed out of the splenic sinuses. There were numerous activated T cells which contained abundant mitochondria and ribosome in the periarterial lymphatic sheaths. The B cells were gradually differentiated into large lymphocytes, immature and mature plasmatic cells with a lot of endoplasmic reticula. They were prominently increased in the splenic nodules. The macrophages had short processes with numerous folds and microvilli and tended to neighboring lymphocytes. The nucleus pores were increased. There were a lot of vacuoles, phagosome and mitochondria were closed to macrophages and lymphocytes. In conclusion the activities of the cellular immunity and humoral immunity were enhanced by laser