冬虫夏草防治氨基糖甙肾毒性损伤的实验研究

Experimental Study on Effect of Cordyceps Sinensis in Ameliorating Aminoglycoside Induced Nephrotoxicity

为阐明冬虫夏草（简称虫草）对氨基糖甙肾毒性损伤的防治作用，我们进行了一系列的实验研究，结果表明：在庆大霉素急性肾损伤模型中，接受虫草治疗的大鼠尿ＮＡＧ酶、血肌酐水平低于对照组，肾小球滤过和保钠功能优于对照组。离体肾灌注（ＩＰＫ）研究证明，虫草可提高ＩＰＫ代谢率，增加肾小球滤过，保护肾小管正常运转。此外虫草还可减轻体外培养的肾小管细胞对庆大霉素损伤的易感性。虫草作用的机理可能包括：（１）拮抗氨基糖甙所致肾脏氧耗下降，提高肾小管Ｎａ＋－Ｋ＋－ＡＴＰ酶活力；（２）减轻氨基糖甙溶酶体损伤和脂质过氧化损伤；（３）降低组织钙含量；（４）通过诱导肾小管细胞ｃ－ｍｙｃ基因表达，以及对损伤状态下肾内肾组织表皮生长因子（ＥＧＦ）调节的保护，促进肾小管的再生修复。

In order to evaluate the effect of Cordycops sinensis(CS)on aminoglycoside (AG) induced nephrotoxicity,gentamycin was imposed on the young and old rats with CS adiministration.The renal tubular injury was ameliorated as evidenced by less prominent increment of BUN,SCr,sodium excretion,urinary NAGase and less severity of histopathological changes as compared with control.In addition,the use of CS could promote an earlier recovery of renal oxygen consumption insulin clearance,and sodium absorption in isolated perfused kidney from CS treated intoxicated rat than that from control.Possible mechanisms of CS on drug-induced nephrotoxicity include:(1)Accelerating the regeneration of tubular cells,(2) Protecting the sodium pump activity of tubular cells,(3)Attenuating the tubular cell lysosome hyperfunction stimulated by phagocytosis of AG as well as decreasing the tubular cell lipoperoxidation in response to toxic injury;(4) Reducing the tissue Ca++ content.