HPLC法测定参茸口服液中人参皂苷Rg1和Rb1含量

Content determination of ginsenoside Rg1 and Rb1 in Renax Oral liquids by HPLC

目的建立参茸口服液中人参皂苷Rg1和Rb1含量测定方法。方法用高效液相色谱法，色谱柱为Waters XTerra^TM Rp18（4．6×250mm 5μm），以流动相A（乙腈）与流动相B（水）进行梯度洗脱；柱温为25℃，检测波长为203nm，流速为1．0mL·min^-1。结果人参皂苷Rg1和Rb1在0．4～4．8μg范围内呈良好线性关系。人参皂苷Rg1平均回收率为99．1％（RSD=1．96％），人参皂苷Rb1平均回收率为97．9％（RSD=2．05％）。结论方法准确，重现性好，可用于该制剂的质量控制。

OBJECTIVE A method for determination of ginsenoside Rg1 and Rb1 in Renax oral liquids by HPLC was created. METHODS The HPLC methods used a Waters XTerra^TM RP18 column,with mobile phase A （acetonitrile） and mobile phase B （water） gradient elution. The column temperature was 25℃ ,the wavelength for detection was 203nm,the flow rate was 1.0mL·min^-1. RESULTS Ginsenoside Rbl and Rg1 showed good linear relationship at the range of 0.4-4.8 μg,the average recovery of Rbl and Ginsenoside Rg1 were 97.9% （RSD=2.05%） and 99. 1%（RSD=1.96%）respectivvely. CONCLUSION The result was accurate and the reproducibility was good. The method can be used for quality control of tinctura.