摘要
目的建立参茸口服液中人参皂苷Rg1和Rb1含量测定方法。方法用高效液相色谱法,色谱柱为Waters XTerra^TM Rp18(4.6×250mm 5μm),以流动相A(乙腈)与流动相B(水)进行梯度洗脱;柱温为25℃,检测波长为203nm,流速为1.0mL·min^-1。结果人参皂苷Rg1和Rb1在0.4~4.8μg范围内呈良好线性关系。人参皂苷Rg1平均回收率为99.1%(RSD=1.96%),人参皂苷Rb1平均回收率为97.9%(RSD=2.05%)。结论方法准确,重现性好,可用于该制剂的质量控制。
OBJECTIVE A method for determination of ginsenoside Rg1 and Rb1 in Renax oral liquids by HPLC was created. METHODS The HPLC methods used a Waters XTerra^TM RP18 column,with mobile phase A (acetonitrile) and mobile phase B (water) gradient elution. The column temperature was 25℃ ,the wavelength for detection was 203nm,the flow rate was 1.0mL·min^-1. RESULTS Ginsenoside Rbl and Rg1 showed good linear relationship at the range of 0.4-4.8 μg,the average recovery of Rbl and Ginsenoside Rg1 were 97.9% (RSD=2.05%) and 99. 1%(RSD=1.96%)respectivvely. CONCLUSION The result was accurate and the reproducibility was good. The method can be used for quality control of tinctura.
出处
《海峡药学》
2007年第1期32-34,共3页
Strait Pharmaceutical Journal