中药仙鹤复方提取物急性毒性试验及对小鼠移植性肿瘤H22的抗肿瘤作用研究

Study on acute toxicity and anti-tumor effect of chinese traditional compound medicine-xianhe

目的研究中药仙鹤复方乙醇提取物的急性毒性，以及中药仙鹤复方乙醇提取物和水提取多糖对小鼠移植性肿瘤H22的抑瘸作用，进而对中药仙鹤复方进行抗肿瘤活性研究。方法测定中药仙鹤复方乙醇提取物的LD50，建立荷实体型肝癌H22小鼠模型，观察中药仙鹤复方乙醇提取物和水提取多糖的抑瘤作用。结果中药仙鹤复方乙醇提取物的LD50为26．95g·kg^-1，95％可信区间为21．99～33．01g·kg^-1；中药仙鹤复方乙醇提取物9．25g·kg^-1·d^-1。剂量组与中药仙鹤复方水提取多糖8．05g·kg^-1·d^-1剂量组对小鼠移植性肿瘤H22均有明显的抑制作用，其抑瘤率分别为49．2％（P〈0．01）和33．7％（P〈0．01）。结论中药鹤复方乙醇提取物和水提取多糖对小鼠移植性肿瘤H22有抑制作用。

OBJECTIVE To observe the acute toxicity of alcohol extracts of chinese traditional compound medicine-Xianhe,and the anti-tumor activity of aqueous extracts and alcohol extracts of Xianhe on transplanted tumor H22 in mice. METHODS The LD50 of alcohol extracts of Xianhe was investigated;Mice of Kunming species which had got the transplanted tumor H22 were used to be experimental model,and antitumor activities were recorded. RESULTS The value of LD50 of alcohol extracts is 26.95g · kg^-1 ,and its range of reliability is 21.99-33.01g · kg^-1 ;The tumor inhibitory rate of alcohol extracts trial group（9.25g · kg^-1· d^-1）and aqueous extracts trial group （8.05g · kg^-1· d^-1 ）were 49.2% （P〈0.01）and 33.7 （P〈0.01）, respectively ; there were both obvious anti-tumorg activities. CONCLUSION In tumor inhibitory test, it was shown that the aqueous extracts and alcohol extracts of Xianhe may possess significantly inhibitory effect on tumor growth of transplanted H22 in mice.