血红素氧合酶在子痫前期胎盘组织中的表达及意义

Study on expression of heme oxygenase in human placenta of preeclampsia

目的探讨子痫前期患者胎盘组织中血红素氧合酶(HO)的两种同工酶蛋白的表达及意义。方法应用免疫组织化学法对20例子痫前期患者(PE组)及20例正常产妇(对照组)胎盘组织中的HO-1和HO-2蛋白进行检测分析,同时测定两组产妇血清HO活性。结果对照组中,HO-1蛋白在胎盘血管内皮细胞的表达量明显低于HO-2蛋白(P<0.05);在胎盘滋养细胞中两者表达量没有差别(P>0.05);PE组胎盘组织中HO-1蛋白的表达与对照组比较,差异无显著性(P>0.05);HO-2蛋白的表达明显降低,与对照组有显著差异(P<0.05);PE组HO活性显著低于对照组(P<0.05)。结论子痫前期胎盘组织中HO-2表达减弱,活性降低,可能是妊娠期高血压疾病发病的机制之一。

[Objective] To investigate the expression and significance of heine oxygenase-1 （HO-1） and heine oxygenase-2 （HO-2） in human placental tissue of preeclampsia. [Methods] Immunohistochemical method was used to examine the expression of HO-1 and HO-2 in placental tissue of preeclampsia （PE, n=20） and normal pregnant women （control group, n=20）. At the same time, the activity of HO in both groups was determined, [Results] In norreal group, the level of HO-1 protein was obviously lower than that of HO-2 protein in villous endothelial cells （P 〈 0.05）; there were no significant difference in trophoblast cells （P 〉0.05）. The level of HO-1 protein in PE group wad no significant difference from that in the control group （P 〉0.05）, but the level of HO-2 protein was obviously lower than that in the control group （P 〈0.05）. The activity of HO in PE group was obviously lower than that in the control group （P 〉0,05）. [Conclusions] The expression level of HO-2 protein is obviously decreased and the activity of HO is degraded in PE group, which may be associated with the pathogenic mechanism of preeclampsia.