月桂酰吲达帕胺脂质体中药物含量及包封率的测定

Quantification and entrapment efficiency determination of lauroyl-indapamide liposomes

目的：建立月桂酰吲达帕胺脂质体中药物含量及包封率的测定方法。方法：采用高效液相色谱法，以Chromasil C18柱为色谱柱；流动相：甲醇-四氢呋喃-0．2％三氟乙酸（pH2．03）（170：15：20）；流速：0．8mL·min^-1；检测波长：240nm；采用SephadexG-50分离游离药物和脂质体，收集并检测脂质体部分药物的含量，计算包封率。结果：月桂酰吲达帕胺在0．01～25ug·mL^-1范围内线性关系良好（r=0．9999），标准曲线为：Y=1．608×10^5 X-5．926×10^2；空白脂质体饱和的SephadexG-50对月桂酰吲达帕胺脂质体的分离效果良好。结论：该方法操作简单，结果准确，适合于该药物脂质体的含量和包封率的测定。

Objective：To establish a quantification method for the determination of the concentration and entrapment efficiency of lauroyl-indapamide in liposomes. Methods： HPLC assay was achieved on a Chromasil C18 column, mobile phase was consisted of methanol- tetrahydrofuran-2% trifloroacetic acid （pH 2.03） （170： 15：20） with a flow rate of 0. 8 mL·min^-1, and detection wavelength was set at 240 nm. The separation of liposomal lauroyl-indapamide from free drug was achieved on a Sephadex G -50 column and the quantification of lauroyl-indapamide was performed based on their peak values. Results：The calibrated linear curve of lauroyl-indapamide concentration was within 0.01 - 25 ug· mL^-1 with the correlation efficiency（r） of 0. 999 9, featuring an equation Y = 1. 608 × 10^5X - 5. 926 × 10^2. The liposomal lauroyl-indapamide was separated well from free drug on the SephadexG -50 column presaturated with the blank liposomes. Conclusion：The simple and accurate method is useable for determination of the concentration and entrapment efficiency of lauroyl-indapamide in liposomes.