亚麻籽胶中的中性多糖NFG-1一级结构的研究

Study on the Primary Structure of NFG-1 from Flaxseed Gum

采用高碘酸氧化、Smith降解、甲基化分析、部分酸水解1、H-NMR和13C-NMR等方法对亚麻籽胶中的NFG-1的结构进行研究。结果表明:NFG-1的主链主要由木糖和葡萄糖组成。大部分阿拉伯糖和部分木糖位于NFG-1的侧链或末端;木糖主要以1→4位键合为主,并存在少量的1→2位键合和1→3位键合,约有1/3的木糖位于非还原末端;葡萄糖主要以1→6或1→2位键合为主;阿拉伯糖有1→4、1→2、1→3位键合,有1/3的阿拉伯糖位于非还原末端;半乳糖存在1→4或1→6位键合,约有1/5位于非还原末端。木糖是β-型,葡萄糖、半乳糖和阿拉伯糖均为α-型。

The structure of NFG-1 was analyzed by method of periodate oxydation, smith degradation, partial hydrolysis, methylation analysis, ^1H and ^13C-NMR. The results showed that the backbone of NFG-1 was composed of xylose and glucose. Most of arabinoses and part of xyloses were in the side-chain or at the end group. Xyloses were mainly of 1→4 linkage, and there were also a few of 1→2 or 1→3 linkage, in which about 1/3 of xyloses were located at the end of unreduced group. Glucoses were mainly of 1→6 or 1→2 linkage. Arabinoses were of 1→4, 1→2 or 1→3 linkage, and about 1/3 of arabinoses were located at the end of unreduced group. Galactoses were of 1→4 or 1→6 linkage, and about 1/3 of galactoses were located at the end of unredueed group. Xyloses were characterized as β-linked glycosidic bond, but for glucose, galactose and arabinose, were α-linked glycosidic bonds.