新疆苏云金杆菌35高效菌株液体深层发酵培养基的筛选

Screening of Formula of Deep-Tank Fermentation Medium for High Effect Bacillus thuringiensis 35

以对棉铃虫高毒力的苏云金芽孢杆菌35作为研究菌株,采用正交试验法及生物测定验证法,对该菌株发酵培养基进行了优化实验。获得了优化培养基(棉籽饼3.25g/dL、玉米粉1.4g/dL、麸皮1.2g/dL、KH2PO40.11g/dL、FeSO40.0049g/dL)。该培养基与对照培养基相比,发酵液含菌数高达40.8×108cfu/mL,比对照培养基提高了63.7%;发酵液毒力高达66.1%,比对照培养基提高了81.5%。

Bacillus thuringiensis （Bt）strain 35 which is highly toxic to Helicoverpa armigera. The orthogonal design has been developed to determine the optimum fermentation medium composition and the culture condition by shaking flasks. The formulas of medium M4 shows higher production than original fermentation medium. The production of spore and bacteria was 725% higher than that of the control fermentation medium in shaker. A optimum fermentation media（the optimized KH2PO4 0. 11 g/dL,FeSO4 0. 0049 g/dL） was achieved. Compared with that of the control media, the concentration of superior media reached 40. 8 × 10^8 cfu/mL, increased 63.7% ; the potency of the optimized media reached 66.1 %, increased 81.5%. The fermentation media is characterized by green environmental protection and this locality and ecology and economy and society.