体外不同胃癌细胞株对表阿霉素敏感性测定的意义

EVALUATION OF THE MTT ASSAY FOR EPIRUBICIN SENSITIVITY TESTING IN HUMAN GASTRIC CANCER CELL LINES IN VITRO

采用MTT方法测定体外MKN－45、SGC-7901、MKN－28胃癌细胞株对不同浓度表阿霉素的敏感性。结果显示0．6μg／ml的表阿霉素对胃癌细胞的抑制率在24小时分别为10.6％、20．9％和11％，到48小时明显增高至43．8％、66.7％和42％（P＜0.01）；表阿霉素浓度增至40μg／ml，72／小时之抑制率进一步上升为78.1％、77.4％和77.3％。分化差的癌细胞对表阿霉素敏感性高于分化好的癌细胞．实验提示，表阿霉素在胃癌化疗时，除考虑肿瘤生物学特性外，还应使用合理的给药方法，以增加与癌细胞接触的浓度和时间，提高有效率。

Epirubicin sensitivity assays were performed with a colorimetric (MTT) assay on MKN-45, SGC-7901 and MKN-28 human gastric cancer cell lines in vitro. The results showedthat from 24h through 48h exposure to 0.6μg/ml of epirubicin, the inhibition rates on tumorcells growth were significantly increased from 10. 6%, 20. 9% and 11% to 43. 8%, 66. 7%and 42 % respectively (P＜0.01). The inhibition rates were still greater (78. 1 %, 77. 4% and77. 3 % respectively,P＜0.01)for 72 h continuous exposure to 40 μg/ml of epirubicin. Poorlydifferentiated carcinoma cells were more sensitive to epirubicin than well differentiated ones.The test indicates that besides the biological behavior of epirubicin, a reasonable administra-tion method should also be considered, so that the concentration and exposure time of epiru-bicin may improve the chemotherapy effectiveness on gastric cancer.