利用微电子细胞传感器阵列动态监测内皮屏障功能(英文)

Label-free and Dynamic Monitoring of Endothelial Barrier Function on Microelectronic Cell Sensor Arrays

内皮在保持血管的动态平衡中起着至关重要的作用,而内皮屏障的功能损伤是很多炎症反应的重要特征。利用实时细胞电子分析系统体外研究脐静脉内皮的屏障功能,从细胞的贴附、生长到融合状态,实时监测了微电子细胞传感器阵列上细胞阻抗的动态变化。α-凝血酶以剂量依赖的方式显著诱导单层内皮细胞阻抗快速下降,而后缓慢回升。相应地,α-凝血酶显著引起细胞单层对荧光标记葡聚糖通透性的改变和F-肌动蛋白细胞骨架的重分布。结果表明,实时细胞阻抗系统可能成为一种体外研究血管内皮细胞形态和屏障功能的有力的手段。

The endothelium plays a vital role in the maintenance of vascular homeostasis and barrier dysfunction is a characteristic of many inflammatory conditions. In our study, an online and continuous technique based on real-time cell electronic sensing （RT-CES） was introduced into study of endothelial barrier function. Electrical impedance in human umbilical vein endothelial cells （HUVECs） was monitored in real-time on microelectronic cell sensor arrays using RT-CES from seeding to confluence. α-thrombin induced electrical impedance alteration in a dose-dependent manner which was paralleled by transendothelial FITC-dextran permeability across endothelial monolayer. Correspondingly, F-actin cytoskeleton redistribution of HUVECs accompanied the change of electrical impedance. The results suggest the RT-CES system could be a powerful tool to study endothelial barrier function model in vitro.