芥蓝不定芽发生过程的基因表达差异分析

Differential Gene Expression Analysis of Chinese Kale during Regeneration by cDNA-AFLP and 2-D Electrophoresis

采用cDNA-AFLP技术和双向电泳技术对不同培养基上的‘中花’芥蓝(Brassicaoleraceavar.alboglabracv.Zhonghua)下胚轴外植体愈伤组织和不定芽发生的基因表达差异进行研究和分析。结果表明:芥蓝不定芽发生基因差异在RNA水平上有所体现,特异条带主要出现在产生不定芽的外植体上,且主要集中在200～600bp之间;芥蓝不定芽发生的基因表达差异在蛋白质水平有很大差异,发生不定芽的外植体和不发生不定芽的外植体出现了160种蛋白质的差异点,差异蛋白的pI值多在5～7之间,在这个范围内的差异蛋白点达到了122个,占差异蛋白总数的76.25%,差异蛋白的分子量多在40～70kDa之间,在这个范围内的差异蛋白点达到了124个,占差异蛋白总数的77.5%。

The differential expression of Chinese kale during regeneration has been analyzed by cDNAAFLP and 2-D electrophoresis. The results indicated that the differential gene expression of Chinese kale adventitious bud occurred at RNA level, the differential band appeared in explants with adventitious bud occurring mainly in 200-600 bp; and the differential gene expression also occurred at protein level, 160 differential proteins have been observed, among which 122 （76.25% of total differential proteins） proteins＇ pI were 5-7. Of the total differential proteins, 124 （77.5% of total differential proteins） proteins＇ MW distributed at 40-70 kDa.