几种不同药物对大鼠全脑缺血再灌注时大脑c－fos基因表达影响的实验研究

Expressionofc┐fosgeneincerebrumduringglobalischemia┐reperfusion

新近研究表明，ｃ－ｆｏｓ基因表达在脑缺血再灌注损伤机制中发挥着第三信使的重要作用。应用ＲＮＡ分子杂交方法，研究尼莫地平等药物对全脑缺血再灌注时大脑ｃ－ｆｏｓ基因表达的影响，以期探讨脑缺血再灌注损伤保护的分子基因机制。缺血前３０分钟静注尼莫地平、安定、硫喷妥钠和吸入异氟醚、安氟醚，全脑缺血再灌注各３０分钟，提取大脑总ＲＮＡ，与ｃ－ｆｏｓＣＤＮＡ探针分子杂交，经过自显影，检测该时点ｃ－ｆｏｓ基因ｍＲＮＡ水平。结果显示：尼莫地平、安定、异氟醚能显著抑制缺血３０分钟再灌注３０分钟ｃ－ｆｏｓ基因表达，而硫喷妥钠、安氟醚对该时点该基因表达无影响。实验提示：ｃ－ｆｏｓ基因参与全脑缺血再灌注损伤保护的分子基因机制。

Itisreportedthattheexpressionofc-fosgenesduringfocalandglobalbrainischemiaandreperfu-sionmayplayanimportantroleasthethirdmessenger.Inthepresentstudy,Weobservedtheefectsoffivedrugsontheexpressionofc-fosgenesformRNA.Nimodipine,diazepineandthiopentalsodiumwereadministeredfor30minpriortoischemiainsult.Inotheranimals,isofluraneandenfluranewerealsogivenbyinhalation.Folowing30minofischemiaandreperfusiontotalcelularRNAofeverycerebrumwasisolated.TheRNAwashybridizedto32P-labeledCDNA.Theresultsindicatedthatnimodipine,diazepineandisofluranedepressedefectivelytheactivationofc-fosgenes.ThiopentalsodiumandenfluranehadnoefectonmRNAofc-fosgene.Theresultssuggestthatc-fosgeneivolvesinmolecularmodulationofcerebraldamageandprotectionglobalbrainreperfusion.