子痫前期肾素血管紧张素系统基因多态性及其相互作用的相关性研究

Relation between genetic polymorphisms of the renin-angiotensin system and pre-eclampsia in Kunming Area

目的探讨血管紧张素转换酶(ACE)基因插入/缺失(I/D),血管紧张素原(AGT)基因M235T,血管紧张素Ⅱ1型受体(AT1R)基因A1166C位点多态性与子痫前期发病的关系。方法采用聚合酶链反应(PCR)和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测58例子痫前期孕妇,102例正常妊娠孕妇的ACEI/D,AGT M235T,AT1R A1166C位点多态性。结果子痫前期组ACEDD型占48.3%(P<0.05,优势比[OR]2.04),II型占8.6%(OR0.34),子痫前期组与对照组相比D等位基因频率显著增加(P<0.01,OR1.9)。AGT M235T,AT1R A1166C多态性与子痫前期无明显相关。子痫前期三个基因的基因型之间无明显协同作用。结论ACE DD型可能增加妊娠期高血压疾病患病风险,ACE I/D,AGT M235T,AT1R A1166C多态性在子痫前期发病危险性上无明显协同作用。

Objective The aim of the present study was to evaluate the relationship between three genetic polymorphisms of the renin-angiotensin system （RAS） and hypertensive disorders complicating pregnancy, methods Fifty-eight hypertensive disorder complicating pregnancy patients and one hundred and two normal pregnant women matched for age and gestational age were evaluated. Genotyping for insertion/deletion of angiotensin-converting enzyme （ACE I/D） , angiotensinogen （AGT） M235T and angiotensin Ⅱ type 1 receptor （AT1R） A1166C polymorphisms were performed using polymerase chain reaction （PCR）, with further restriction fragment length polymorphism （RFLP） analysis when required. Results 48.3% of patients were homozygous for DD （P 〈 0.05, odds ratio [OR] 2.04） ; and only 8.6% of patients carried the Ⅱ genotype （OR 0.34） . Patients had a significantly higher D allele frequency （P 〈 0.01, OR 1.9） than did the control. No significant association was found for （AGT） M235T and AT1R A1166C. There was no preferential interaction between ACE and AGT or AT1R genotypes in hypertensive disorders complicating pregnancy patients. Conclusions Our findings suggest that an unfavorable genetic pattern of ACE may contribute to the increased risk for the development of hypertensive disorders complicating pregnancy.