摘要
目的:建立测定健康受试者血浆样品中佐米曲普坦浓度的液相色谱-质谱(HPLC-MS)定量分析方法。方法:血浆样品经乙腈直接沉淀并在真空泵中挥干后,利用 HPLC-MS/MS 选择性离子检测。结果:本法线性范围为0.1~40ng·mL^(-1),最小检出浓度为0.05 ng·mL^(-1),日内、日间 RSD 均小于15%。结论:本法可以用于测定血浆中佐米曲普坦浓度,方法准确、灵敏、快速,重现性好。
Objective:To establish an HPLC - MS method for determination of zolmitriptan in human plasma. Methods:The plasma sample was deproteinated by acetonitrile, and after it was dried by the vacuum pump, the sample was injected into the HPLC - MS/MS system. Result: The linear range of zolmitriptan was 0. 1 - 40 ng ·mL^-1, and the detection limit was 0.05 ng ·mL^-1. The intra and inter day RSD of zolmitriptan were all less than 15%. Conclusion:The assay method is shown to be suitable for determining the concentration of zolmitriptan in plasma. This method is accurate, sensitive, rapid and reproducible.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第2期171-173,共3页
Chinese Journal of Pharmaceutical Analysis