胶束电动毛细管色谱法分离分析三七中的人参皂苷Rg_1、人参皂苷Re和三七皂苷R_1

Separation and determination of ginsengnoside Rg_1,ginsengnoside Re and notoginsengnoside R_1 from Radix et Rhizoma Notoginseng by micellar electrokinetic chromatography

目的:用胶束电动毛细管色谱法分离测定三七中人参皂苷 Rg_1、人参皂苷 Re 和三七皂苷 R_1 3种皂苷含量。方法:采用Beckman MDQ 电泳仪,40 cm×50 μm的熔融石英毛细管,有效柱长30 cm,缓冲液为20 mmol·L^(-1)SDS、40 mmol·L^(-1)胆酸钠、60 mmol·L^(-1)硼砂,检测波长200 nm。结果:人参皂苷 Rg_1低、中、高剂量的平均加样回收率分别为101.5%,100.6%,100.4%,RSD 分别为2.4%,2.2%,1.0%;人参皂苷 Re 低、中、高剂量的平均加样回收率分别为101.3%,99.23%,99.66%,RSD 分别为2.0%,1.3%,1.1%;三七皂苷 R_1低、中、高剂量的平均加样回收率分别为100.6%,100.5%,100.7%,RSD 分别为1.1%,1.1%,0.86%。结论:本方法能够将三七药材中人参皂苷 Rg_1、人参皂苷 Re 和三七皂苷 R_1完全分离,具有灵敏度高、快速、易于重现且所用试剂价格低廉的特点,因此适合于中药三七的测定,并对今后三七的鉴别和质量控制具有重要意义。

Objective：To separate and determine ginsengnoside Rgl, ginsengnoside Re and notoginsengnoside R1 from Radix et R_hizoma Notoginseng. Method：Experiments were performed on Beckman P/ACE MDQ capillary electrophoresis system,the fused -sillica capillary tube was used with 20 mmol · L^-1 SDS,40 mmol · L^-1 sodium cholata,60 mmol · L^-1 Na2B4O7. The dimersion of the capillary columns was 50 μm ×40 cm,30 cm from the inlet to the detection window,on - line UV detector was operated at 200 nm. Results： Under optimum condition, the recoveries of the analytes in the extact of Radix notoginseng in low, medial, high level was 101.5%, 100. 6%, 100. 4% and the RSD was 2. 4% ,2. 2% ,1.0% for ginsengnoside Rg1 ,in low,medial,high level was 101.3%, 99. 23% ,99. 66% ,and the RSD was 2.0% ,1.3% ,1.1% for ginsengnoside Re,and in low,medial,high level was 100. 6% ,100. 5% ,100. 7% ,and the RSD was 1.1% ,1.1% ,0.86% for notoginsengnoside R1 respectively. Conclusion ：The optimum method puts up a good reproducibility, high accuracy and great linear, which meets the request of analysis. The results show that the method can he used to control quality of Radix et Rhizoma Notoginseng.