期刊文献+

HPLC法同时测定曲氯乳膏中醋酸曲安奈德和氯霉素的含量 被引量:7

Simultaneous HPLC determination of triamcinolone acetonide acetate and chloramphenicol in triamcinolone acetonide acetate and chloramphenicol cream
下载PDF
导出
摘要 目的:建立高效液相色谱法同时测定曲氯乳膏中氯霉素和醋酸曲安奈德的含量。方法:用 Hypersil C_(18)(250mm×4.6mm,5 μm)色谱柱;以甲醇-水-正己烷(65:35:2)为流动相;流速为1.0 mL·min^(-1);柱温为20℃;检测波长为240 nm。结果:氯霉素和醋酸曲安奈德的保留时间分别为4.54 min 和16.17 min,理论塔板数分别为2650和6500;HPLC 法测定的线性范围分别为51.32~975.08μg·mL^(-1)(r=0.9999,n=5)和3.116~46.74μg·mL^(-1)(r=0.9999,n=5);回收率分别为100.5%~102.3%(RSD<1.1%)和98.8%~101.7%(RSD<1.5%)。结论:本法用于曲氯乳膏中氯霉素和醋酸曲安奈德的含量测定,简便、快速、准确、可靠。 Objective :To establish an HPLC method for determination of triamcinolone acetonide acetate and chloramphenicol in triamcinolone acetonide acetate and chloramphenicol cream at the sametime, the Qulv cream and establish the quality control method. Methods:The column:ZORBAX SB -C18 (4. 6 mm 250 mm,5 μm) ,the mobile phase was methanol - water - n-hexane (65: 35: 2 ) and the flow rate was 1.0 mL · min^- 1. The column temperature was 25 ℃ and the detection wavelength was 240 nm . Results:The retention times for triamcinolone acetonide acetate and chloramphenicol were 4. 54 min and 16. 17 min, respectively and there were good linear relationship within the range of 51.32 - 975.08 μg · mL^-1 ( r = 0. 9999, n = 5 ) and 3. 116 - 46. 74 μg · mL^-1 ( r = 0. 9999, n = 5 ). The recoveries of chloramphenicol and triamcinolone acetonide acetate were 100. 5% - 102.3% ( RSD 〈 1.1% ) and 98. 8% - 101.7 % (RSD 〈 1.5 % ), respectively. Conclusion :The method is convenient, rapid, accurate and reliable and may be adopted for assay of triamcinolone acetate and chloramphenicol in triamcinolone acetonide acetate and chloramphenicol cream.
出处 《药物分析杂志》 CAS CSCD 北大核心 2007年第2期272-274,共3页 Chinese Journal of Pharmaceutical Analysis
关键词 曲氯软膏 醋酸曲安奈德 氯霉素 高效液相色谱法 triamcinolone acetonide acetate and chloramphenicol cream triamcinolone acetonide acetate chloramphenicol HPLC
  • 相关文献

参考文献3

二级参考文献4

  • 1[1]Akay C,Ozkan S.A,Senturk Z,et al .Simultaneous determination of metronidazole and miconazole in pharmaceutical dosage forms by RP - HPLC[J ] . Ⅱ Farmaco, 2003, 57(11): 953.
  • 2.化学药品地方标准上升国家标准.Vol 2(第二册).[S].,2002.67.
  • 3.中国药典(二部)[M].,2000.846.
  • 4段莲华,李玉丽,郭晶,乔风敏,王兴彦.HPLC法测定皮康霜软膏中的醋酸曲安奈德[J].药物分析杂志,2002,22(4):321-323. 被引量:12

共引文献76

同被引文献26

引证文献7

二级引证文献35

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部