摘要
目的:建立高效液相色谱法同时测定曲氯乳膏中氯霉素和醋酸曲安奈德的含量。方法:用 Hypersil C_(18)(250mm×4.6mm,5 μm)色谱柱;以甲醇-水-正己烷(65:35:2)为流动相;流速为1.0 mL·min^(-1);柱温为20℃;检测波长为240 nm。结果:氯霉素和醋酸曲安奈德的保留时间分别为4.54 min 和16.17 min,理论塔板数分别为2650和6500;HPLC 法测定的线性范围分别为51.32~975.08μg·mL^(-1)(r=0.9999,n=5)和3.116~46.74μg·mL^(-1)(r=0.9999,n=5);回收率分别为100.5%~102.3%(RSD<1.1%)和98.8%~101.7%(RSD<1.5%)。结论:本法用于曲氯乳膏中氯霉素和醋酸曲安奈德的含量测定,简便、快速、准确、可靠。
Objective :To establish an HPLC method for determination of triamcinolone acetonide acetate and chloramphenicol in triamcinolone acetonide acetate and chloramphenicol cream at the sametime, the Qulv cream and establish the quality control method. Methods:The column:ZORBAX SB -C18 (4. 6 mm 250 mm,5 μm) ,the mobile phase was methanol - water - n-hexane (65: 35: 2 ) and the flow rate was 1.0 mL · min^- 1. The column temperature was 25 ℃ and the detection wavelength was 240 nm . Results:The retention times for triamcinolone acetonide acetate and chloramphenicol were 4. 54 min and 16. 17 min, respectively and there were good linear relationship within the range of 51.32 - 975.08 μg · mL^-1 ( r = 0. 9999, n = 5 ) and 3. 116 - 46. 74 μg · mL^-1 ( r = 0. 9999, n = 5 ). The recoveries of chloramphenicol and triamcinolone acetonide acetate were 100. 5% - 102.3% ( RSD 〈 1.1% ) and 98. 8% - 101.7 % (RSD 〈 1.5 % ), respectively. Conclusion :The method is convenient, rapid, accurate and reliable and may be adopted for assay of triamcinolone acetate and chloramphenicol in triamcinolone acetonide acetate and chloramphenicol cream.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第2期272-274,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
曲氯软膏
醋酸曲安奈德
氯霉素
高效液相色谱法
triamcinolone acetonide acetate and chloramphenicol cream
triamcinolone acetonide acetate
chloramphenicol
HPLC