4种氟喹诺酮类药物在肉鸡组织中残留的研究

Simultaneous Determination of Four Fluoroquinolone Residues in Broilers Tissues

采用高效液相色谱法同时检测肉鸡肌肉、脂肪、肝脏和肾脏组织中4种氟喹诺酮类药物（FQS）的残留。检测条件为：采用HP-StableBond C18分析柱（5um，250mm×4．6mm），以15mmol／L的四丁基溴化铵溶液-乙腈（体积比94：6）为流动相（用乙酸钠缓冲液调pH3．6），流速1．0ml／min，柱温30℃；采用荧光检测器检测，激发波长280nm，发射波长460nm。测定结果表明，该方法对FQS的最低检测限为0．92ug／kg，在5．00～100．00ug／mg的线性范围内，溶液含量与峰面积的相关系数达0．9998以上。在高、中、低3种含量水平下对所测肉鸡组织中4种FQS进行回收率测定，结果为74％～99％，相对标准偏差低于6．5％，该方法能同时测定4种FQS在动物组织中的残留，具有简便、准确、灵敏度较高的特点，符合痕量测定的要求。

An high performance liquid chromatographic method with a fluorescence detector was established to determine four fluoroquinolone residues in broilers tissues. The chromatographic conditions were as follows： HP- StableBond C18 column （5 um,250 mm×4.6 mm） was used at 30℃ with 15 mmol//L tetrabutylammonium bromide solution （pH3.0）- acetonitrile （94：6,v/v） as the mobile phase with a flow rate of 1.0 ml/min, At the excitation wavelength of 280 nm and emission wavelength of 460 nm . The detection limit for broilers tissues was 0. 92 ug/kg. The linear range was from 5.00-100.00 ug/mg, And correlation coefficients were more than 0.9998. At the levels of 10, 50 and 100 ug/kg, The recoveloped can meet the requirement for residue analysis.