鸭生长激素基因5-’UTR和3-’UTR多态性分析

Polymorphism Analysis on 5'-UTR and 3'-UTR of Duck Growth Hormone Gene

作者根据生长激素基因5’-UTR和3’-UTR的序列设计5对引物,用PCR-SSCP的方法对北京鸭、西湖野鸭、樱桃谷鸭、金定鸭、山麻鸭、荆江鸭、绍兴鸭、缙云麻鸭等8个品种共436个样本进行了单核苷酸多态性分析,并在第1对引物中检测到了多态性。对2种类型的片段纯化测序,结果发现在5’-UTR第230位和244位碱基发生了突变,分别由C突变为G、由C突变为A。但只在金定鸭、荆江鸭、绍兴鸭中发现多态,统计分析结果显示金定鸭的AB基因型频率显著高于荆江鸭和绍兴鸭,且金定鸭A等位基因的频率也显著高于荆江鸭和绍兴鸭。

The single nucleotide polymorphism （SNP） of GH gene was investigated in various breeds, including beijing ducks, xihu mallards, cherry valley meat ducks, jinding ducks, shan partridge ducks, jingjiang ducks, shaoxing ducks, jinyun partridge ducks. Five primers for 5＇-UTR and 3＇-UTR of GH gene were designed according the database of duck genomic sequence and the SNPs were detected by PCR-SSCP method. Polymorphism （C/G at 230 and C/A at 244 ） was only found among individuals within jinding ducks, jingjiang ducks, shaoxing ducks. Population genetics analysis showed that the frequency of AB genotype in jinding ducks was significantly higher than that of jingjiang ducks and shaoxing ducks and the frequency of A allele in jinding ducks was significantly higher than that in jingjiang ducks and shaoxing ducks.