丢糟中微量成分提取方法的研究

Study on Trace Components Extraction Methods in Distiller’s Grains

采用CO2超临界萃取法和酒精热浸提法提取丢糟中的微量成分,通过气相色谱分析其主要酯类物质的含量,结果表明:(1)两种方法均未能提取丢糟的乙酸乙酯;(2)CO2超临界萃取法提取了丢糟的乳酸乙酯、己酸乙酯、丁酸乙酯和戊酸乙酯4种乙酯,而酒精热浸提法只有乳酸乙酯和戊酸乙酯2种乙酯,且后者乳酸乙酯的含量275.92855mg/100g远远大于前者的11.89551mg/100g。因此,就提取丢糟中主要酯类成分的效果来看,CO2超临界萃取法优于酒精热浸提法。

Two methods including supercritical fluid extraction （CO2-SFE） method and 60% alcohol heat extraction method were used to extract trace components. The contents of main component in the extract were determined by gas chromatography （GC）. The results showed that： （1） Ethyl acetate can not be extracted by the two methods; （2）Four kinds of ester including ethyl lactate, ethyl caproate, ethyl butyrate and ethyl valproate can be extracted by CO2-SFE, but only two kinds of esters including ethyl lactate and ethyl valproate can be abstracted by 60% alcohol heat extraction, with the ethyl lactate content about 275. 92855mg/100g much higher than 11.89551 mg/100g extracted by CO2-SFE. Based on the above results, it is suggested that the CO2- SFE method is better than heat extraction method.