冬虫夏草液体发酵产多糖条件优化及饮料研制

Study on Optimization of Submerged Fermentation for Cordycepic Polysaccharides and Beverage Exploitation

研究了培养时间、初始pH值、乙醇浓度、二次补糖等因素对冬虫夏草菌丝生长和产糖的影响,得出了最佳培养时间为6d,初始pH为5.5,乙醇浓度为1.0%,二次补糖为复合碳源葡萄糖+甘露糖+半乳糖(3:4:2)1.2g/100ml,发酵液胞外多糖产量和菌丝干重最高分别可达5949.6mg/L和17.35g/L。功能性虫草饮料以添加枸杞+党参(2:1)15g/100ml发酵的配伍最佳。最优饮料配方:发酵液:水=70:30、蔗糖7%、柠檬酸0.1%、稳定剂0.15%。

The effects of culture time, initial pH, ethanol concentration and secondary supplementing sugar on the biomass and the polysac, charides production of Cordyceps sinensis were studied. The results showed：the optimum culture time 6d, initial pH 5.5, ethanol thickness 1.0% and secondary supplementing sugar Glu＋Man＋Gal（3：4：2）1.2g/100ml. The maximal output of polysaccharides and the mycelium weight reached 5949.6mg/L and 17.35g/L respectively. The optimum functions obtained when cordycepic beverage was added with Lycium barbarum and Codonopsis pilosula （2：1） 15g/100ml. The best beverage formula is fermentative fluid：water=-70：30, sucrose 7%, citric acid 0.1% and stability 0.15%.