大豆异黄酮和牛初乳复合制剂对去卵巢大鼠骨密度及子宫组织抗氧化作用的研究

Effects of Complexes of Soy Isoflavones and Bovine Colostrum (SIBC) on Bone Density and Anti-oxidant of Uterus in OVX Rats

目的:探讨大豆异黄酮和牛初乳制剂(复合制剂)对去卵巢大鼠骨密度及子宫超氧化物歧化酶(SOD)活性、丙二醛(MDA)和谷胱甘肽(GSH)含量的影响。方法:大鼠去卵巢建立骨质疏松症模型,连续灌胃给予复合制剂三个月,测定胫骨骨密度及骨钙、磷、锌含量,测定子宫MDA、GSH含量、子宫SOD活性。梯度胶电泳分离子宫SOD同工酶,分析SOD谱带活性。结果:大鼠建模后,骨密度及骨钙、磷、锌含量均低于正常对照组,给予复合制剂后,各剂量组骨密度和骨钙、磷、锌含量均升高,其中高、中剂量组的骨重、骨密度、骨矿盐密度和中剂量组的骨钙密度与模型相比,差异有显著性;中、低剂量组的骨钙、磷和低剂量组的骨锌,显著高于模型组。去卵巢大鼠子宫MDA含量明显升高,GSH含量显著下降,给予复合制剂后,中剂量组MDA含量下降明显,GSH含量增加,与模型组比差异有显著性。模型组子宫SOD活性显著低于对照组,给予复合制剂后能显著提高中剂量组子宫SOD比活性、Mn-SOD活性和高剂量组SOD比活性。大鼠子宫有三种SOD同工酶,去卵巢后同工酶活性都受到了明显抑制,给予复合制剂后,中、低剂量组的SOD同工酶活性恢复明显。复合制剂含有类SOD活性为3492U/g。结论:复合制剂能增加去卵巢大鼠骨密度,抑制骨量丢失,提高子宫组织抗氧化损伤能力,对骨质疏松症有较好的改善作用。

Objective： To investigate the effects of the complexes of SIBC （soy isoflavones and bovine colostrum） on BMD（bone mineral density）, and SOD（superoxide disnmtase）, MDA（malonaldehyde） and GSH（glutathione） concentration of uterus in ovariectomized rats. Methods： The osteoporosis model was induced by extirpating uterus of rats and the rats were given SIBC for 3 months. The tibias bone density and contents of calcium, phosphorus and zinc and MDA and GSH concentrations of uterus were all assayed by spectrophotometry. SOD activity was assayed after the extract of uterus brought to 25% saturation with （NH4）2SO4. The isozyme of uterus SOD activity was analyzed by 8%-37% concentration gel gradient electrophoresis. Results： Compared with model group, SIBC groups significantly increase BMD and concentrations of calcium, phosphorus, zinc; decrease the concentration of MDA and increase the concentration of GSH in uterus. The SOD activity in the model group is lower than the control group. The specific activity of SOD both in the middle dose group and high dose group and activity of Mn-SOD in the middle dose group also increase after given SIBC. The activity of the isozyme markedly decreases in the model group, but after given SIBC, the activity of isozyme of middle dose group and low dose group markedly increase. The SOD-like activity of SIBC is 3492U/g. Conclusions： SIBC could increase BMD and prevent bone loss and could increase the anti-oxidation of uterus in OVX（ovariectomy） rats. It showed that SIBC has improvement on osteoporosis disease.