当归多糖对亚急性辐射损伤小鼠的防护作用研究

Study on Radioprotection Effects of Angelica sinensis Polysaccharides on Sub-acute Radiation-injured Mouse

目的:考察当归多糖ASP3组分对亚急性辐射损伤小鼠的防护作用。方法:以当归多糖ASP3组分为受试物,用60Coγ射线对小鼠进行一次性全身照射,采用推片染色、试剂盒检测、MTT等方法检测外周血白细胞、淋巴细胞数量,骨髓嗜多染红细胞(PCE)微核率,淋巴细胞转化率,肝组织SOD活力、MDA含量。结果:ASP3对亚急性辐射损伤小鼠的外周血白细胞、淋巴细胞数量的回升均有明显的促进作用,并能有效地抑制PCE微核的形成,促进外周血淋巴细胞转化以及肝组织的抗氧化能力,增强机体的辐射耐受性。结论:当归多糖具有一定的抗辐射功能,对亚急性辐射损伤小鼠有良好的防护作用。

Objective： To investigate the radioprotective effect of Angelica sinensis polysaccharide ASP3 on sub-acute radiationinjured mice. Methods： Polysaccharide ASP3, prepared from Angelica sinensis, was orally administered to groups of mice. After being exposed wbolely to ^60Coγ-rays once, peripheral leucocyte and lymphocyte counts, micronuclei frequency of PCE in bone marrow, transformation rates of peripheral lymphocyte, SOD and MDA of liver cells were examined. Results： ASP3 could protect leucocytes and lymphocytes, prohibit formation of micronucleus of polychromatic erythrocytes in bone-marrow, speed transformation rate of peripheral lymphocytes, content of SOD of liver cells and enhance the radiation endurance of the body. Conclusion： ASP3 has radioprotective effects on sub-acute radiation-injured mice.