摘要
采用流通池光纤荧光光度法，对固定化乳酸脱氢酶（ＬＤＨ）在静磁场作用下的催化活性进行了探讨．发现在０．０８４～０．２３０Ｔ磁场作用下ＬＤＨ的催化活性明显降低，其降低值随磁场强度增强而增大，且与磁场作用的时间和ｐＨ值有关，而在２５～４０℃范围内，温度则无显著影响。
The catalytic activity of immobilized LDH in static magnetic field has been investigated using fiber optical fluorimetry with flow cell.Remarkable decrease of the activity of LDH is observed in a magnetic intensity range of 0.084 to 0.230 T.Asthe magnetic intensity is increasing until up to 0.230 T.the activity of LDH is decreasing until by-17%.This effect is related to magnetization time and pH of subtrate solution, while temperature has no effect in the range of 25-40℃
出处
《湖南大学学报（自然科学版）》
EI
CAS
CSCD
1996年第5期57-61,共5页
Journal of Hunan University:Natural Sciences
基金
国家自然科学基金
关键词
磁场
乳酸脱氢酶
光纤荧光光度法
催化活性
magnetic field,lactate dehydrogenase,fiber optic fluorimerty,catalytic activity.
