高效液相色谱法测定血浆甲硝唑浓度

Determination of Serum Metronidazole Concentration by HPLC

目的建立血浆甲硝唑浓度的高效液相色谱（HPLC）测定方法。方法采用HPLC-UV方法，以法舒地尔为内标；色谱柱为Hypersil C18柱（5μm，4．6mm×200mm）；流速：1．0mL·min^-1；柱温：30℃；流动相：0．02mol·L^-1磷酸二氢钠溶液（用1mol·L^-1磷酸溶液调节pH值=3．00）：甲醇=80：20，紫外检测波长319nm。结果甲硝唑的血药浓度在0．25-20．00mg·L^-1范围内与峰面积比有良好的线性关系，定量下限为0．25mg·L^-1。方法的平均回收率为（98．24±1．30）％，日内、日间相对标准差（RSD）均〈10％。结论该方法简单、方便、准确，是血浆甲硝唑浓度检测较好的分析方法。

Objective To establish a HPLC method for determination of the serum metronidazole concentration. Methods A HPLC-UV assay was developed. The interstandard was fasudil. The determinate wavelength was 319nm. The colum was Hypersil C18 （5 μm, 4. 6 mm × 200 mm）. The mobile phase was KH2PO4-methnol （80 ： 20, V/V） , and the column temperature was 30℃. The flow rate was 1.0 mL·min^-1. Results The determination limit was 0. 25 mg·L^-1. The metmnidazole was linear in the range from 0. 25 mg·L^-1 to 20.0 mg·L^-1. The RSD was no more than 10.0%. Conclusion The HPLC method is simple, convenient and accurate, which provides a reference method for determination of serum metronidazole concentration.