电针对全脑缺血再灌注大鼠脑组织氨基酸递质的影响

Effect of Electroacupuncture on Excitatory Amino Acid in Brain Tissue of Rat with Cerebral Ischemia Reperfusion

目的:研究电针对全脑缺血再灌注损伤脑组织兴奋性氨基酸递质(EAA)的影响。方法:四动脉阻断法造成SD大鼠全脑缺血再灌注损伤模型,高效液相色谱法测定脑组织谷氨酸(GLU)、天冬氨酸(ASP)和甘氨酸(GLY)含量,原子吸收分光光度计测定脑组织Ca2+含量,干湿重法测定脑组织含水量。结果:与假手术组比较,模型组脑组织GLU、ASP、Ca2+含量和含水量明显升高,GLY含量无明显变化;循经取穴电针能显著降低升高脑组织GLU、ASP、Ca2+含量和含水量,与模型组比较有显著性差异,对GLY含量无明显影响;穴位对照组和非穴位对照组脑组织GLU、ASP、GLY、Ca2+含量和含水量与模型组比较,均无显著性差异。结论:电针治疗脑缺血再灌注损伤的机制之一与降低EAA兴奋性毒性有关,并具有一定的穴位特异性。

Objective ：To observe the effects of dectroacupuncture（EA） on excitatory amino acid（EAA） in brain tissue after cerebral ischemia reperfusion（CIR）. Methods： CIR model in rat was established by Pulsinelli＇s Four Vessel Occlusion method. The contens of glumatate（Glu）, asparate（Asp）, glycine（Gly） in brain tissue were analyzed by high performance liquid chromatography with fluorescent detection. Atomic spectrophotometry was used to measure Ca^2＋ content. The changes of brain water content（BWC） were measured by the wet and dry weight methods. Results： At 120 rain after perfusion, the contents of Glu, Asp, Ca^2＋ and water in brain tissue increased. EA along the Meridian could decrease the contents of Glu, Asp, Ca^2＋ and water in brain tissue, but no significant difference in acupoint control group and non - acupoint control group, compared with model control group. Conclusion： The theraputic action of EA on CIR can be realized by lowering EAA neurotoxicity and has acupeint specificity in some degree.