基于导电聚合膜及MEMS技术的安培免疫传感器

Micro Amperometric Immunosensor Based on Conducting Polymer and MEMS

研究采用微电子机械系统（MEMS）技术在硅基底上制备可集成微型安培免疫传感器，用于人免疫球蛋白（IgG）的检测．该传感器包括两个铂电极以及SU-8胶形成的微反应池和敏感池．工作电极的敏感表面首先聚合聚吡咯膜，然后通过戊二醛的交联作用实现抗体的固定．最后通过夹心法实现对IgG的检测．该传感器检测线性范围5-255ng／mL，响应时间3min具有线性好、响应快、试剂用量少、微型化、便于集成等优点．

A micro amperometric immunosensor is fabricated on silicon wafer for Immunoglobulin G （IgG） using the technique of Micro-Electro-Mechanical Systems （MEMS）. The immunosensor is composed of two electrodes made of platinum and two micro cells （reaction cell and sensing cell） made of SU-8. Firstly, polypyrrole is electropolymerized on the electrode. Then antibody is crosslinked by glutaraldehyde. IgG is specifically recognized and scaled in sandwich way. The immunosensor displays a linear dose-response behavior for human IgG concentrations between 5 and 255 ng/mL and a 3-min response time. This micro amperometric immunosensor has many advantages such as good linearity, fast response time, little reagent, micro bulk and easy integration with IC.