有机质谱学在蛇床子素、厚朴酚、和厚朴酚与鱼腥草素结构确定中的应用

Applications of Organic Mass Spectrometry to Structure Elucidation of Osthole,Magnolol,Honokiol and Houttuynium

有机质谱学在中药药效物质的结构确定中起到了至关重要的作用.对中药药效物质质谱的确切解释需要对这些药效物质质谱的关键裂解规律有足够的了解.应用有机质谱学规律对中药药效物质的结构确定进行了讨论.这些中药的药效物质包括:应用超临界二氧化碳从蛇床子中提取的蛇床子素和从厚朴中提取的厚朴酚与和厚朴酚;应用水蒸汽蒸馏从鱼腥草中提取的鱼腥草素等.具有较高丰度的质谱关键碎片峰m/z229的出现是蛇床子素质谱的典型特征;质谱关键碎片峰m/z169的出现是鱼腥草素质谱的典型特征.而质谱关键碎片峰m/z247是否出现则是区分厚朴酚与和厚朴酚的质谱典型特征.在此基础上,尝试构建中药药效物质的有机质谱学平台.

Organic mass spectrometry plays a crucial role in the structure elucidation of those effective substances from traditional Chinese medicine. Secure interpretation of the mass spectra of the effective substances from traditional Chinese medicine requires an adequate understanding of the mechanism of the key fragmentation processes of the mass spectra of the effective substances. The present paper discussed the application of the regular patterns of organic mass spectrometry to the structure elucidation of the effective substances from traditional Chinese medicines. These effective substances from traditional Chinese medicine include osthole from Cnidium monnieri, magnolol and honokiol from Manolia officinalis extracted by supercritical CO2 method; houttuynium from Houttuynia cordata extracted by hydro-distillation. The pres- ence of the key fragment peak at m/z 229 with higher abundance is the typical characteristic of the mass spectrum of osthol and the presence of the key fragment peak at m/z 169 is the typical characteristic of the mass spectrum of houttuynium. But the presence or absence of the key fragment peak at m/z 247 is the typical characteristic of the mass spectra to differentiate the chemical structures between magnolol and honokiol. We are trying to construct the platform of organic mass spectrometry for effective substances from traditional Chinese medicine based on this study.