啤酒发酵过程中野生酵母污染检验新方法研究

New Method for Inspection of Contamination Yeasts in the Process of Brewage

提取培养酵母TY-1和野生酵母W1,W2的基因组DNA,进行Delta-PCR,可以分别得到稳定而独特的DNA指纹图谱。把TY-1,W1和W2按比例混合,提取混合菌基因组DNA,进行Delta-PCR。结果表明:混合菌的Delta-PCR指纹图谱是纯菌图谱的叠加。通过对凝胶电泳图谱中特征带的分析,可以看出:即使混合菌体系中仅含有0.05%的野生酵母也能被Delta-PCR技术检出。

Genomic DNA specific primers of delta of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with sequence. Each strain showed the stable and unique DNA fingerprint when PCR products were analyzed in gel electrophoresis. TY-1, W1 and W2 were mixed proportionately, and examined by Delta-PCR. The result showed that the DNA fingerprints of the mixture were the superposition if each pure yeast strains. Analysis of the main bands showed that contamination yeast can be examined by Delta-PCR even if the mixed yeast system contained 0.05% of contamination yeast.