IL-1β反义RNA在肝癌细胞中对NK细胞固有免疫杀伤的影响

IL-1β Antisense RNA in HepG2 Hepatoma Cells Affected NK Cells Mediated Cytotoxity

目的:探讨将所获IL-1β反义RNA真核表达载体导入H epG 2细胞后,肝癌细胞对NK细胞杀伤敏感性的变化。方法:转染后用RT-PCR法检测反义基因片段的表达,M TT比色法分析NK-92细胞杀伤活性的变化。结果:转染后反义RNA均得到高水平表达,并使NK细胞的杀伤活性提高约15%。结论:IL-1β反义RNA可在一定程度上恢复肝癌细胞对NK细胞的杀伤敏感性。

Objective：To study the variation of NK cells mediated cytotoxicity against transfected HepG2 hepatoma cells by IL- 1β antisense RNA eukaryocyte expression vectors. Methods：After the transfection, RT-PCR was performed to detect the expresstion of the two antisense gene segments, and MTT colormetric assay was executed to analyze NK cells mediated cytolysis activity against HepG2 cells. Results：After the transfection, antisense RNA had been successfully expressed respectively, and NK cells activity bad been improved about 15%. Conclusion：To some extent IL-1β antisense RNA can revertothe sensitivity of HepG2 cells to NK cells mediated cytotoxicity.