巨噬细胞分泌FIZZ1刺激血管平滑肌细胞增殖

Effect of IL-4 on expression of macrophage FIZZ1 and proliferation of smooth muscle cell

目的探讨IL-4对巨噬细胞FIZZ1分泌及其FIZZ1对血管平滑肌细胞增殖的影响。方法用不同剂量的重组IL-4刺激培养的巨噬细胞,RT-PCR检测巨噬细胞内FIZZ1 mRNA表达,荧光免疫组化激光共聚焦显微镜检测巨噬细胞FIZZ1蛋白表达;用终浓度分别为3×10-6mmol/L,9×10-6mmol/L,2.7×10-5mmol/L的FIZZ1刺激培养的平滑肌细胞,MTT法测定FIZZ1对平滑肌细胞增殖的影响。结果Th2型细胞因子IL-4刺激巨噬细胞,FIZZ1 mRNA及其蛋白明显表达;浓度为3×10-6mmol/L,9×10-6mmol/L,2.7×10-5mmol/L的FIZZ1明显促进平滑肌细胞增殖(与对照组比较P<0.05),且随着FIZZ1剂量的增加,促增殖作用增强。结论Th2型细胞因子IL-4能刺激巨噬细胞表达FIZZ1 mRNA及其蛋白,FIZZ1能促进体外培养的血管平滑肌细胞增殖。

Objeetive To explore the effect of IL-4 on the expression of macrophage FIZZ1 and FIZZ1 on the proliferation of smooth muscle cell. Methods After collected mouse peritoneal macrophages, cultured macrophages were stimulated by different dose of recombinant IL-4. C57BL/6J mouse thoracic aorta were taken and divided into 3 fragments. When cell growed to the 5th generation,cultured cells were stimulated by normal sodium（control groups ） and different dose of recombinate FIZZ1 （ final concentration 3 × 10^-6 mmol/L, 9 × 10^-6 mmol/L, 2.7× 10^-5 mmol/L）. The proliferation of smooth muscle cell was detected with the methods of MTT. Results When IL-4 stimulated macrophages, FIZZlmRNA and protein expressed significantly;FIZZ1 promoted the proliferation of smooth muscle cell in a dose -dependent manner（FIZZ1 groups vs control groups,P〈0.05）. Conclusion IL-4 induces macrophages FIZZlmRNA and protein expression; FIZZ1 promots the proliferation of smooth muscle cell.