红三叶总异黄酮对人乳腺癌MCF-7细胞增殖和凋亡的影响

Effect of red clover total isoflavones on cell proliferation and apoptosis of human breast cancer cell line MCF-7

目的研究红三叶总异黄酮对人乳腺癌MCF-7细胞的生长抑制和凋亡的诱导作用。方法在体外细胞培养下,采用不同质量浓度红三叶异黄酮和5-Fu处理MCF-7细胞,MTT法测定红三叶总异黄酮抑制MCF-7细胞增殖的量-效关系;TUNEL法、流式细胞术分析其细胞凋亡率和周期分布。结果红三叶总异黄酮对MCF-7细胞增殖的抑制作用随药物质量浓度提高和作用时间延长而更加明显,而在低质量浓度时却出现轻微促细胞增殖现象。TUNEL法、流式细胞术分析结果表明,红三叶总异黄酮能诱导MCF-7细胞凋亡,阻滞细胞于G2/M期,降低细胞增殖指数,且这种作用也存在剂量-效应关系。结论红三叶总异黄酮可抑制MCF-7细胞增殖并诱导其凋亡,具有抗肿瘤作用。

Objective To study the effect of red clover total isoflavones on proliferation and apoptosis of human breast cancer cell line MCF-7 and the molecular mechanism of the action. Methods Proliferation of MCF-7 cells pre- and post-treatment with red clover total isoflavones and 5-Fu was determined by MTT assay. The induction of apoptosis and the change of cell cycle in MCF-7 cells treated with red clover total isoflavones were investigated by TUNEL and Flow cytometry. Results MTT Assay showed that the proliferation of MCF-7 cells in vitro was inhibited by red clover total isoflavones in time-dependent and concentration-dependent manners. But it could promote the proliferation in low concentration （〈25.0μg/mL）. TUNEL and flow cytometry showed that the apoptosis and inhibition of MCF-7 cells in G2/M phase were induced after treated with red clover total isoflavones. Conclusion The total isoflavones from red clover have the effect of anti-tumor for they could inhibit the proliferation and induce the cell apoptosis of MCF-7 cells.