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青牛胆SRAP标记反应体系的建立与优化 被引量：7

Establishment and optimization of SRAP reaction system in Tinospora sagittata
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摘要 目的为青牛胆的物种鉴定和遗传图谱的构建寻找一种新的途径。方法采用序列相关扩增多态性(Sequence related amplified polymorphism,SRAP)技术对青牛胆DNA进行PCR扩增,逐级优化反应参数。结果优化得到稳定重复性好的青牛胆SRAP反应体系。结论SRAP技术在分子水平上对青牛胆进行鉴定是一种行之有效的手段,为今后进一步的青牛胆物种鉴定、遗传图谱的构建等研究奠定基础。 Objective To develop a new method for Tinospora sagittata species identification and genetic map construction. Methods Sequence-related amplified polymorphism （SRAP） was applied for T. sagittata to carry on PCR amplification of its DNA and optimize the reaction parameter grade by grade. Results The stable and reproducible SRAP reaction system of T. sagittata has been developed. Conclu- sion SRAP is an effective method for T. sagittata identification in molecular degree and it has set up a foundation for the further species identification and genetic map construction of T. sagittata.
作者 杨兵 王天志 罗禹 陈璐
机构地区 四川大学华西药学院
出处 《中草药》 CAS CSCD 北大核心 2007年第2期272-275,共4页 Chinese Traditional and Herbal Drugs
关键词 青牛胆 SRAP 分子标记 Tinospora sagittata （Oliv.） Gagnep sequence-related amplified polymorphism （SRAP） molecular marker
分类号 R282.7 [医药卫生—中药学]
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参考文献6

  • 1Li G, Quiros C F. Sequence-related amplified polymorphism(SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theor Appl Genet, 2001, 103: 455-461.
  • 2Riaz A, Li G, Quresh Z, et al. Genetic diversity of oilseed brassica napu inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance[J].Plant Breed, 2001, 120(5): 411-415.
  • 3林忠旭,张献龙,聂以春,贺道华,吴茂清.棉花SRAP遗传连锁图构建[J].科学通报,2003,48(15):1676-1679. 被引量：205
  • 4武志朴,杨文香,刘大群,张汀.小麦基因组SRAP扩增体系的初步研究[J].河北农业大学学报,2005,28(3):66-69. 被引量：56
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二级参考文献20

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二级引证文献33

中草药
2007年 第2期

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