基质固相分散-高效液相色谱法同时测定烟草中的有机酸

Determination of six organic acids in tobacco by high-performance liquid chromatography based on matrix solid phase dispersion

研究了用基质固相分散-高效液相色谱法测定烟草中有机酸的方法。将烟草样品与石墨化碳黑球(W/W,1∶4)充分研磨后装柱,用热水洗脱,洗脱液中的有机酸用高效液相色谱测定。以ZORBAX Stable Bound(4.6×50mm,1.8μm)快速分离柱为固定相,0.01mol/LNaH2PO4(pH2.98)和乙腈(体积比为98:2)为流动相,流速为2.0mL/min,柱温25℃,检测波长210nm。方法回收率为94%～98%;相对标准偏差为2.1%～3.0%;检出限为80～200μg/L。色谱分离时间不超过3.0min。用于实际样品分析,结果令人满意。

A new method for the determination of organic acids in tobacco based on matrix solid phase dispersion was established. Samples and graphitized carbon black sorbent （ 1 ： 4） were homogenized in a mortar for 5 min and then packed into a cartridge. Organic acids were eluted from the cartridge with water and then analyzed by high-performance liquid chromatography with 2% methanol （containing 0.01 mol/L NaH2PO4 buffer, pH 2.98） as mobile phase at a flow rate of 2.0 mL/min and detected at wavelength of 210 nm. The 6 organic acids were separated on a ZORBAX Stable Bound （4.6 x 50 mm, 1.8μm） column within 3 min. Limits of detections （S/N = 3） were below 200 μg/L. Mean recoveries ranged from 94% to 98%, and relative standard deviations were below 3.0 %. This method has been successfully applied to the determination of organic acids in tobacco with satisfactory results.