糖皮质激素受体α和受体β与特发性血小板减少性紫癜糖皮质激素抵抗的关系

A study on the relationship between α and β-isoform of glucocorticoid receptors and glucocorticoid in resistant idiopathic thrombocytopenia purpura.

目的探讨糖皮质激素受体α(GRα)和糖皮质激素受体β(GRβ)在原发性血小板减少性紫癜(ITP)糖皮质激素抵抗中的分子生物学机制。方法选择2003-01—2005-12在深圳市儿童医院血液内科住院的ITP患儿30例,将患儿分为2组:激素敏感组(18例)和激素抵抗组(12例),另选10例正常儿童作对照。应用RT-PCR法检测激素敏感组、激素抵抗组ITP患儿及10例正常儿童外周血单个核细胞GRα和GRβmRNA的表达水平,以探讨GRα和GRβ与ITP糖皮质激素抵抗的关系。结果(1)对照组、激素敏感组和激素抵抗组3组之间GRαmRNA的表达[分别为(0.75±0.17)、(0.70±0.15)、(0.73±0.16)]差异无显著性,P>0.05;(2)对照组和激素敏感组的GRβmRNA[分别为(0.13±0.03)、(0.15±0.04)]差异亦无显著性,P>0.05,但激素抵抗组的GRβmRNA水平[为(0.39±0.12)]显著高于对照组和激素敏感组,P<0.01,差异具有统计学意义。结论ITP患儿糖皮质激素抵抗可能与GRβmRNA的表达亢进有关。

Objeαive To identify the relationship between the expression of α and β-isoforms of glucocorticoid receptors （GR） and response to glucocorticoid in the patients with idiopathic thrombocytopenia purpura （ITP）. Methods Semiquantitative RT-PCR was used to deteα the expression of GRα, β in PBMC among 30 patients with ITP and 10 normal controls. Results （ 1 ） The expression of GRαmRNA in PBMC showed among the groups. （ 2 ） The expression of GRβmRNA in the resistant group of ITP was significantly higher than that in both the sensitive group and control group （ P 〈 0. 01 ）. Conclusion The expression of GRβmRNA may play a great role in resisting glucocorticoid resistance in the patients with ITP.