革兰阴性菌L型与其原菌内毒素含量比较

Comparison of endotoxin content between Gram negative bacteria and their L-forms

目的:探讨革兰阴性菌L型和原菌内毒素含量差异。方法:采用定性和定量鲎试验,分别检测稳定的大肠埃希菌和铜绿假单胞菌L型及其原菌内毒素含量。结果:大肠埃希菌和铜绿假单胞菌L型内毒素定性鲎试验检测阳性,定量鲎试验检测出大肠埃希菌和铜绿假单胞菌L型内毒素含量分别为原菌内毒素含量的25.1%和36.4%。结论:革兰阴性菌L型仍能产生内毒素,但其含量较原菌明显减少,是其致病力减弱的原因之一。

Objective：To compare endotoxin content between Gram negative bacteria and their L-forms. Methods：The endotoxin content was detected by qualitative and quantitative limulus tests in E. coli and P. aeruginosa and their stable L-forms. Results：The qualitative limulus tests of Gram negative bacterial L-forms were positive. The endotoxin content of E. coli L-forms is 25.1% of their parental bacteria, and P. aeruginosa L-forms was 36.4% of parental. Conclusions： Gram negative bacterial L-forms generate less endotoxin.